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Cell Analytics Facility

Research Area: 
Life & Medical Sciences
Location: 

Bizkaia Science and Technology Park
Building 205 E-48170 Zamudio (Spain)

Contact: 

(+34) 946 018 135
info@achucarro.org

Available for external users?: 
Yes
Access protocol: 

For further details, please contact the center.

Description: 

The Cell Sorting Unit is equiped with a BD FACSJazz (2B/4YG) cell sorter and analyser. This cell sorter incorporates some new design features (intuitive alignment, real-time video monitoring, drop-delay confirmation,…) that simplify the operation of stream-in-air machines, which helps increasing the operational efficiency for high workloads.

The Facility also hosts a Extracellular Flux Analyzer.

Equipment

Cell sorter and analyser

Optics
-488 nm (blue) laser and 561 nm (yellow-green) laser independently aligned.
- 20X, 0.6NA microscope objective.
- Forward Scatter Detector and Filters: 75- and 50-mm lenses and PMT tube with a 488/10 BP filter and OD3 neutral density filter.
- Side Scatter Detector and Filter: 90º collection lens and 488/10BP filter PMT.
- Fluorescence Detectors and Filters: 90º collection lens and PMTs (2B/4YG).