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ADVANCED LIGHT MICROSCOPY PLATFORM

Keywords: 
Microscopy, Biophysics, Biochemstry

ADVANCED LIGHT MICROSCOPE

Research Area: 
Physical Sciences
Life & Medical Sciences
Location: 

Unidad de Biofísica (Biophysics Unit)
Campus Universitario de Leioa (University of the Basque Country UPV/EHU)
Barrio Sarriena s/n
48940 Leioa, Vizcaya (Spain)

Contact: 

Unai Lorenzo
email: ulorenzo001@ehu.eus

Dr. Jose Requejo-Isidro
email: Jose.Requejo@csic.es

Available for external users?: 
Yes
Description: 

Technical description of the instrumentation
• Multiphoton scanning microscope: Leica SP5, Spectra-Physics Mai-Tai (pulsed Ti:sapphire laser tunable from 690 to 1040nm)
• Incubator and chamber for living cell imaging during extended periods
• High quantum efficiency GaAsP hybrid detectors and APDs for sensitive detection
• TCSPC card: Becker & Hickl SPC 830 (FLIM, FCS and FCCS)
• Analysis software: Imaris and Huygens. In-house developed analysis software
• Storage and management of large volume of images: OMERO

Services: 

Applications of interest
• Spatio-temporal study of molecular interactions on the single cell basis (FRET-FLIM)
• Intact-cell imaging of lipid concentration at the plasma membrane
• Ligand-receptor binding (FCS)
• Lipid-mediated protein interactions (scanning FCS)
• Lipid packing in a membrane (Generalised Polarisation)