Platform for Biomarkers

Infrastructures
Research areas
  • Physical Sciences
Contact

Aitor Palomino  (aitor.palomino@achucarro.org)

Available for external users?
Yes
Access protocol

Please contact our staff

Description

Single Molecule Array platform

Our Quanterix SIMOA HD-X analyzer is a fully automated instrument for running immunoassays using single molecule array technology (SIMOA), a digital form of ELISA, trapping and sealing individual immunocomplexes on paramagnetic beads in thousands of femtoliter sized wells in arrays found on the SIMOA discs. By leveraging the digital detection format, this technology enables to precisely measure protein biomarkers with substantially higher sensitivity allowing the application of precision science to precision health.

Main technical specifications

  • Throughput of 3 plates every 8 hour shift. About 300 data points per day.
  • Workflow, batch (plates or tubes)
  • Total assay time is less than 2.5 hours per 96-well plate
  • Hands-on time, startup time <20 minutes
  • Sample input, 96-well plate and tubes
  • Sample volume, 1 µl * - 100 µl
  • Multiplex capability, up to 4-plex
  • Assay flexibility, 1, 2 or 3-step assays, with variable incubation time and number of wash steps

 

Benefits

  • Ultra-sensitivity – Up to 1000x greater sensitivity than traditional immunoassays
  • Automation – Reproducibility and convenience of sample->answer workflow
  • Multiplexing – Measure up to 4 biomarkers in single sample at fg/ml concentrations
  • Flexibility – Supports both highthroughput and rapid result workflows
  • Efficiency – Precise control of assay conditions allows for use of stored assay calibration values
  • Menu – Fully compatible with 80+ assays as well as customized homebrew assays

     


Location

Achucarro Basque Center for Neuroscience Bizkaia Science and Technology Park Building 205 E-48170 Zamudio (Spain)